Aims: To determine the chemical toxicity of cadmium using tube bioluminescence extinction method and measuring the extent to which light production by the bacteria is inhibited in realtion to the amount of cadmium concentration in 10 lipstick samples.
Study Design: The design of the study is experimental method.
Place and Duration of Study: Microbiology-Parasitology Department of UPHSL-Binan, Laguna and SGS Philippines, Inc., Makati City, between September to October 2018.
Methodology: The researchers isolated Vibrio fischeri, a marine luminous microbe, from a local squid, cultured and was identified using biochemical test. A A small part was chipped from each lipstick sample and placed in different test tubes containing the bioluminescent bacteria with 3% saline solution and dispensed by two-fold serial dilution, 10 ml in several tubes. A fast and qualitative luminescence measurement was done by swirling the tube in a completely dark room and the intensity of bioluminescence was observed and recorded. The amount of cadmium present in lipstick samples was detected using Atomic Absorption Spectrophotometry (AAS() as the control test ion cadmium.
Results: The results of the study showed that in terms of inhibition period of bioluminescence, lipstick J has the shortest time interval with an average of 86.0 minutes and lipstick B has the longest time interval with an average of 221.7 minutes. However, the result of the AAS showed that there was no presence of cadmium in lipstick samples A, B, C, D, E, F, G, H, I, and J respectively.
Conclusion: Furthermore, based on the study conducted, inhibition period biolumenescence differs significantly across lipstick samples with different toxicants. The inhibition period of bioluminescence is highly associated with the heavy metals content in lipsticks. Lower heavy metals content leads to longer inhibition periods and vice versa.